The activation of extracellular signal-regulated kinases 1 and 2 by insulin-like growth
factor I in human osteosarcoma MG-63 cells was examined by Mono Q ion exchange chromatography
of cell extracts and measurement of myelin basic protein kinase activity, and by immunoblotting
of cell extracts with a phospho-specific extracellular signal-regulated kinase antibody.
Extracellular signal-regulated kinase 1 appeared to be activated in resting cells
and addition of insulin-like growth factor I resulted in the activation primarily
of extracellular signal-regulated kinase 2. Extracellular signal-regulated kinase
2 was found in the nucleus after addition of insulin-like growth factor I.
Insulin-Like Growth Factor I Receptor - Mono Q Ion Exchange Chromatography - Nuclear
Extracellular Signal Regulated Kinase 2
